HotelBeds is cutting about 5% of its global workforce, with cuts in Tel Aviv, London, Dubai, Orlando and Zurich.
The decision, it says, is part of an "optimization" plan.
The Spain-based bedbank says it has now consolidated into one business following its acquisitions in early 2017 of Tourico Holidays and GTA.
It is now focusing on becoming the most "efficient bedbank player in the industry," it says in a statement.
Announcing results for the year ending September 30, the company reported EBITDA of €233.5 million (compared to €207.9 million year-over-year) and a cash position of €498 million.
HotelBeds also launched a strategy to reduce "cost-per-room-night" from €6.30 currently to €4.90 in the next two years.
In a statement announcing the results and job cuts, the company says it plans to "invest in automation and cutting-edge technology" to improve the customer experience and make better use of the unified business.
HotelBeds recently pushed back on speculation in the Spanish press that is an acquisition target with tech giant Amadeus and OTAs including Booking.com and Expedia in the frame.
It has also announced the opening of a new technology hub in Valencia, Spain to develop future platforms and processes.
The company was sold by TUI to Cinven and CPPIB for €1.2 billion in April 2016.