Ryanair will no longer distribute fares to travel agencies via Amadeus after the pair were unable to reach agreement on a new contract.
The pair have worked together since September 2014.
The European low-cost carrier says it has existing contracts with Travelport and Sabre and will continue to allow their subscriber travel agencies to book tickets on the carrier.
It says the commercial agreements with both remain unchanged and it has no comment to make with regards to its commitment to the travel agency channel for selling fares.
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Ryanair will also continue to use fare filing and passenger service system provider, Navitaire, which is now owned by Amadeus.
The Ireland-based airline made a fairly triumphant return to third party distribution in March 2014, initially with Travelport, then Amadeus and Sabre coming later the same year.
It was the first time in a decade that Ryanair had agreed to give travel agencies the ability to buy fares through GDS.
The policy in the intervening ten years had been to drive sales through its website - a strategy that had also seen it tangle with a number of so-called "screen scrapers".
CEO Michael O'Leary had often - and typically - made fairly disparaging remarks about the travel agency channel, such was the company's policy to be a direct-only carrier.
Yet the move to change course in 2014 and enlist the help of GDSs once more to help distribute content was considered by many as a smart one given its desire to attract more business travellers.
Ryanair also had ambitions at the time to hit the 119 million passenger mark by 2019, coinciding with the purchase of 175 new aircraft.
Ryanair says in a statement:
"We wish Amadeus well and thank them for their support during our successful GDS partnership."
An Amadeus official adds: "We wish Ryanair well and look forward to continuing our partnership through Navitaire, an Amadeus company, as Ryanair is a Navitaire New Skies customer."