Duetto can boast $143.2 million in funding rounds to date after landing a huge Series D investment of $80 million this week.
The hotel revenue management and analytics provider has attracted the support of funds belonging to Warburg Pincus, it says in a statement.
The latest funding dwarfs the last round of $30 million from Icon Ventures in August 2015 - a round that was 50% larger than the Series B from Accel Partners in July 2014.
The company claims it is the largest ever "non-acquisition" investment in a hotel software company.
It currently has more than 2,500 hotels and casinos from 60 countries on its books, boasting that customers are beating their competitors to the tune of 6.5% growth since adopting its array of products.
The company has a number of products in the market, including the core revenue management system to handle conversion strategy and other distribution strategies, an optimization tool for hotel channels, consulting service, reporting platform, and A/B testing system.
The latest investment will be used as part of its growth plan to scale-up the business and develop additional tools for hoteliers.
CEO and co-founder, Patrick Bosworth, says: "By unifying data across the tech stack, our platform has become the single source capable of delivering true personalization to consumers.
"Hoteliers now have the ability to increase conversion and drive more direct engagement with consumers by personalizing pricing and merchandising at the point of booking."
Warburg Pincus has previously invested in the likes of Avaloq, Helix, Green Cross, Mobike and Ucar.
Fellow co-founder and chief marketing and strategy officer, Marco Benvenuti, says the company wants to build on its "open and agnostic" strategy by working with fellow technology companies.
* Check out this clip from The Phocuswright Conference 2017, featuring Bosworth
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