G Adventures will use a growth investment round from Certares to help with mergers and acquisitions for the group tour operating business.
The amount of capital put into the 31-year-old company is not being detailed.
It is only the second time in the brand's history that it has disclosed external funding, although both occasions were via private equity agreements.
The new injection of capital (the last being in 2010) will be used for the M&A activity and investment in technology and the platform.
Founder and CEO Bruce Poon Tip, will retain a controlling share of the company, says: "I have always said tourism can be the biggest form of wealth distribution the world has ever seen and this investment means we can do even more to build back travel the right way."
The Certares investment is the second for the private equity group this week, after leading a $42 million Series C round into cabin rental platform Getaway.
* Check out this interview with founder and managing director Greg O'Hara, recorded at The Phocuswright Conference 2020.
Executive Interview: Rolling the Dice - Greg O'Hara, Certares - The Phocuswright Conference 2020